Product details

NadPrep rRNA Blocking Reagent

Catalog Number:

#1002901#1002902
#1002911#1002912

NadPrep rRNA Blocking Reagent is a specialized processing module developed for high-throughput sequencing platforms to rapidly block rRNA in total RNA. This kit-series offer both commercial and customized solutions, supporting species-specific designs. It is suitable for total RNA with initial input amounts ranging from 50-500 ng, sourced from cells, tissues, and FFPE samples. If you require a customized design for a specific species, please contact us at (support@njnad.com) for professional, personal advice.

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Feature

Simple and Convenient

Mix the rRNA Blocking Reagent directly with the reaction system in a single pipetting step

Fast and Efficient

Efficiently block rRNA in just 8 min
Effectively enrich low-abundance RNA
Enhance informative content and reduce sequencing costs

Flexible Compatibility

Compatible with samples of different RNA quality
Support the input ranging from 50 to 500 ng of RNA sample
Compatible with third-party RNA library preparation kits
Flexible customizition for extension to other species or regions

Workflow

Performance

Compatible with Different Input Amounts for Various Sample Types

Figure 1. Performance of different input amounts across various types of samples using NadPrep rRNA Fast Blocking Comprehensive Solution (Human). A. rRNA ratio; B. No. of genes detected; C. Transcript coverage. Libraries were prepared using NadPrep rRNA Rapid Blocking Comprehensive Solution connected to NadPrep DNA Library Preparation Kit-series. Sequencing platform: Illumina® Novaseq 6000, PE150, and 0.3 Gb of data per sample was randomly selected for data analysis.
Note: The Cell line is Human Brain Total RNA (Takara, 636530); DV 200 values for FFPE A and FFPE B are 69% and 32%, respectively.

Figure 2. Performance of different input amounts of total RNA from zebrafish using NadPrep rRNA Fast Blocking Comprehensive Solution (Zebrafish). A. rRNA ratio; B. No. of genes detected; C. Transcript coverage. Libraries were prepared using NadPrep rRNA Rapid Blocking Comprehensive Solution (Zebrafish) connected to NadPrep DNA Library Preparation Kit-series.

Compatible with Different Grades of FFPE Samples

Figure 3. Performance of different grades of FFPE samples using NadPrep rRNA Fast Blocking Comprehensive Solution (Human). A. rRNA ratio; B. No. of genes detected; C. Transcript coverage.
Note: For samples derived from FFPE with an input of 250 ng, the DV 200 values are 72%, 69%, 61%, 57%, 48%, and 32%, respectively.

Compatible with Third-party RNA Library Preparation Kits

Figure 4. Performance of NadPrep rRNA Rapid Blocking Comprehensive Solution (Human) in combination with third-party RNA library preparation kits. A. rRNA ratio; B. No. of genes detected; C. Transcript coverage; D. Expression profile correlation. A total of 250 ng of K562 cell line RNA were processed using NadPrep rRNA Blocking Reagent in conjunction with NadPrep Total RNA-To-DNA Module, followed by library preparation using NadPrep DNA Library Preparation Kit-series, KAPA and NEB RNA Library Prep Kits. A total of 50 ng of zebrafish RNA was processed using NadPrep rRNA Blocking Reagent (Zebrafish) in conjunction with NadPrep Total RNA-To-DNA Module, followed by library preparation using NadPrep DNA Library Preparation Kit-series, Yeasen and ABclonal RNA Library Prep Kits.
Note: H: Human; Z: Zebrafish.

Compatible with MGI and Illumina Sequencing Platforms

Figure 5. Performance of NadPrep rRNA Fast Blocking Comprehensive Solution (Human) on dual sequencing platforms. A. rRNA ratio; B. No. of genes detected. With an input of 250 ng, libraries were prepared using NadPrep rRNA Fast Blocking Comprehensive Solution connected to NadPrep DNA Library Preparation Kit-series, coupled with NadPrep Universal Stubby Adapter (UDI) Module and NadPrep Universal Adapter (MDI) Module respectively. Sequencing platform: DNBSEQ-T7 and Illumina Novaseq 6000, PE150.

Competitive Comparison

Figure 6. Performance comparison between NadPrep rRNA Fast Blocking Comprehensive Solution and competitors. A. rRNA ratio; B. No. of genes detected; C. Transcript coverage; D. Expression profile correlation. With an input of 250 ng, libraries were prepared using NadPrep rRNA Blocking Reagent, Vendor A, and Vendor B in combination with NadPrep Total RNA-To-DNA Module.
Note: Vendor A refers to the rapid removal method, and Vendor B refers to the RNase H enzymatic method. C & D are shown only with the cell line (K562 RNA).

For research use only. Not for use in diagnostic procedures.

RNA-To-DNA Module

Package	Color of Cap Tube	Component	Volume 1002401 (24 rxn)	Volume 1002402 (96 rxn)
Box1		1^st Strand Synth. Buffer	120 µL	460 µL
		Random Primer	30 µL	115 µL
		1^st Strand Synth. Enzyme	60 µL	230 µL
		2^nd Strand Synth. Buffer	810 µL	2×1550 µL
		2^nd Strand Synth. Enzyme	90 µL	345 µL
	/	Nuclease Free Water	2.5 mL	8 mL
Box2	/	NadPrep SP Beads	2.5 mL	10 mL

rRNA Blocking Reagent (Human)

Color of Cap Tube

Component

Volume

1002901 (24 rxn)

Volume

1002902 (96 rxn)

rRNA Blocking Reagent (Human)

30 µL

115 µL

rRNA Blocking Reagent (Zebrafish)

Color of Cap Tube

Component

Volume

1002911 (24 rxn)

Volume

1002912 (96 rxn)

rRNA Blocking Reagent (Zebrafish)

30 µL

115 µL

For research use only. Not for use in diagnostic procedures.

Is the NadPrep rRNA Fast Blocking Comprehensive Solution suitable for severely degraded FFPE samples?

Title: Is the NadPrep rRNA Fast Blocking Comprehensive Solution suitable for severely degraded FFPE samples?

A: This solution is suitable for RNA samples from a wide range of cell and tissue sources with varying degradation levels. However, it is not recommended for severely degraded FFPE samples (DV 200 < 20%).

Is the NadPrep rRNA Fast Blocking Comprehensive Solution compatible with other third-party RNA library preparation kits?

Title: Is the NadPrep rRNA Fast Blocking Comprehensive Solution compatible with other third-party RNA library preparation kits?

A: Yes, it is. This solution can be integrated with RNA library preparation kits for the preparation of total RNA sequencing (RNA-Seq) libraries. If you choose a third-party RNA library preparation kit, we recommend using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB, Cat # E7770L) or the KAPA RNA HyperPrep Kit (Roche, Cat # KK8541). For the use of RNA library preparation kits from other brands, please consult technical support from Nanodigmbio with support@njnad.com.

Why is the dsDNA yield slightly higher when prepared using the NadPrep rRNA Fast Blocking Comprehensive Solution?

Title: Why is the dsDNA yield slightly higher when prepared using the NadPrep rRNA Fast Blocking Comprehensive Solution?

A: This solution contains a large amount of rRNA ssDNA blocking reagent. When used in conjunction with the NadPrep Total RNA-To-DNA Module, there may be a slight residual of ssDNA in the purified dsDNA product. When quantifying dsDNA using a nucleic acid fluorescence quantifier, this residual ssDNA can influence the results, leading to a slightly higher yield. However, the remaining rRNA ssDNA blocking reagent does not affect subsequent experiments. We recommend appropriate adapter dilution factors and PCR amplification cycles for different different types of RNA and different input amounts.

Type	Product	Detail	Catalog#
rRNA Fast Blocking Module	NadPrep rRNA Blocking Reagent (Human), 24 rxn	24 rxn	1002901
	NadPrep rRNA Blocking Reagent (Human), 96 rxn	96 rxn	1002902
	NadPrep rRNA Blocking Reagent (Zebrafish), 24 rxn	24 rxn	1002911
	NadPrep rRNA Blocking Reagent (Zebrafish), 96 rxn	96 rxn	1002912
Total RNA-To-DNA Module	Nadprep Total RNA-To-DNA Module, 24 rxn	24 rxn	1002401
Total RNA-To-DNA Module	Nadprep Total RNA-To-DNA Module, 96 rxn	96 rxn	1002402