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HLAtyping Panel v2.0

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#1001952 (16 rxn)
#1001951 (96 rxn)

HLAtyping Panel v2.0 refers to the latest database (Version 3.50), and targets 11 HLA genes (Class I: HLA-A, B, C; Class II: HLADPA1,DPB1, DQA1, DAQB1, DRB1/3/4/5). The probe covers the full-length genome sequence of all alleles and supports high precision typing to the fourth field level.

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Performance

Capture Performance

Figure 1. Capture performance of HLAtyping Panel v2.0. Following targeted capture of standards using HLAtyping Panel v2.0, sequencing was performed using Illumina^® platform.
Note：The standards are Class I and Class II UCLA DNA Reference Panel. The capture performance was reflected through calculated mean values and standard deviations of 24 Class I and 24 Class II.

Genotyping results

Table 1. Genotyping results for Class I by HLAtyping Panel v2.0.

Libraries were prepared using 50 ng of gDNA and the NadPrep EZ DNA Library Preparation Kit v2, hybrid capture (6 plex) was completed using HLAtyping Panel v2.0, followed by sequencing using NovaSeq 6000 PE150 with the data of approximately 0.5 Gb. Sequencing data were HLA typed using in-house tools.
Note：The standard is Class I UCLA DNA Reference Panel. The first row of each standard refers to the typing result for the standard and the second row refers to the data typing result using HLAtyping Panel v2.0.

Figure 2. Coverage of reference sequence under non-mismatching conditions with targeted capture for C1-213 using HLAtyping Panel v2.0. The sequence data for typing results was aligned to the reference sequence of the type. Individual results with differences in the second field level presented in the typing results of Class I using HLAtyping Panel v2.0. After sequence alignment and inspection, it was found that the new typing results using HLAtyping Panel v2.0 were more in line with the situation: The whole reference sequence showed continuous coverage under non-mismatching conditions; while the old results showed interruption of coverage, that is, the sequence composition here lacked support。

Note：C*08:02 (top) is the typing result provided by standard C1-213, with discontinuous coverage; C*08:01 (middle) is the new typing result, with continuous coverage; meanwhile, the coverage of another allele C*12:02 with consistent results (bottom) is shown.

For research use only. Not for use in diagnostic procedures.

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Volume

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1001951

HLAtyping Panel v2.0, 96 rxn

415 μL

–20℃

1001952