Product details

NadPrep IGTR Multiplex PCR Library Prep Kit

Catalog Number:

#1300001
#1300002

NadPrep IGTR Multiplex PCR Library Prep Kit is based on the principle of multiplex amplification, enabling efficient enrichment of the complementary determining regions (CDR3) of seven loci for human adaptive immune antigen receptors, including IGH, IGK, IGL, TRA, TRB, TRG and TRD. Combined with high-throughput sequencing technology, this kit facilitates precise detection of B-cell and T-cell diversity and clonality. It is compatible with human DNA or cDNA initial samples from various sources and is widely applicable for immune repertoire studies, clonality analysis of lymphoid malignancies, and minimal residual disease (MRD) monitoring.

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Feature

● Modular Primer Design

Provides two independent primer sets: IG Primer Mix (covering IGH/IGL/IGK gene-specific primers) and TR Primer Mix (covering TRA/TRB/TRG/TRD gene-specific primers).
Supports single-tube independent detection (IG or TR only) or dual-tube combined detection (IG + TR).
● Broad Compatibility
Sample types: Compatible with human gDNA or cDNA (first-strand cDNA generated from RNA via reverse transcription).
Input flexibility: Compatible with input amounts ranging from 50 to 2,000 ng.
Sequencing platforms: Direct sequencing on Illumina® platforms or sequencing on MGI platforms after circularization.

Workflow

Performance

Flexible Combination of IG/TR Primer Mixes in Separate Tubes for Precise Amplification of Target Regions

Spiking 0.001% of a mixture of RAMOS and JURKAT cell line gDNA into 2 μg leukocyte-derived gDNA, libraries were prepared using NadPrep IGTR Multiplex PCR Library Prep Kit, with independent amplification (IG-only or TR-only) or combined amplification (IG + TR).

Table 1. Clone list generated by analysis tool.

Figure 1. CDR3 diversity analysis using the NadPrep IGTR Multiplex PCR Library Prep Kit. A. IG Primer Mix (single tube)(on-target: 86.49%); B. TR Primer Mix (single tube) (on-target: 93.36%); C. IG Primer Mix + TR Primer Mix (dual tubes) (on-target: 91.62%).
Note: Sequencing was performed on NovaSeq 6000 (PE150) with data of 2~2.4 Gb for analysis.

#Package	Cap Color	Component	Volume 1300001 (6 rxn)	Volume 1300002 (96 rxn)
Box 1		2X Multiplex PCR Mix	180 μL	2×1,600 μL
		IG Primer Mix	15 μL	230 μL
		TR Primer Mix	15 μL	230 μL
		Multiplex Index Primer Mix #	6×5 μL	24×10 μL
Box 2	/	NadPrep SP Beads	400 μL	6,400 μL

Type	Product	Detail	Catalog#
Multiplex PCR Lib Prep	NadPrep IGTR Multiplex Library Prep Kit, 6 rxn	Index #1-6	1300001
Multiplex PCR Lib Prep	NadPrep IGTR Multiplex Library Prep Kit, 96 rxn	Index #1-24	1300002