Target Capture Solution for Pan-Cancer Solid Tumor Based on NadAuto-96 Automatic Sample System

Background

The application of high-throughput sequencing technology is becoming more and more extensive, and the demand of laboratory for large-scale, high-efficiency, and high-stability experimental operations is becoming more and more urgent. Compared with manual operation, the solution of automated workstations has obvious advantages in many aspects. Nanodigmbio has developed the NadAuto-96 Automatic Sample System, which truly realized a one-stop automated target capture solution of "instrument + reagent + application".

仪器特点-英文版2

Solution

The libraries were prepared from 50 ng Human Genomic gDNA (Male) standard (Promega, G1471).

The automated process uses an on-instrument application to build 96 libraries/batch; 500 ng of each library for 1-plex, 4-plex, 8-plex hybrid capture; Manual operation shall be carried out according to the corresponding product protocol.

Library Preparation	Blocking	Target Capture
NadPrep EZ DNA Library Preparation Module v2, 96 rxn	NadPrep NanoBlockers（for MGI, SI), 96 rxn NadPrep NanoBlockers（for MGI, DI), 96 rxn	NanOnco Plus Panel v3.0, 96 rxn NadPrep Hybrid Capture Reagents, 96 rxn

For research use only. Not for use in diagnostic procedures

Library Preparation on NadAuto-96

Figure 1. Comparison of library yield between automated and manual operation.

Figure 2.Size Distribution of libraries were prepared on NadAuto-96.

Note: 12 libraries were selected and detected by Qseq.

Figure3. The difference of library yield between different batches prepared on NadAuto-96.

Hybridization Capture on NadAuto-96

The libraries prepared on NadAuto-96 were mixed according to different multiplexing scheme, and then continued hybridization capture on NadAuto-96.

Figure 4. The relative captured library yield of NadAuto-96.

Capture Performance of different multiplexing scheme on NadAuto-96 as follows:

Figure 5. Capture performance of different multiplexing scheme on NadAuto-96.

Figure 6: Size distribution and GC bias of captured libraries with 1-plex.

Figure 7: Size distribution and GC bias of captured libraries with 4-plex.

Figure 8: Size distribution and GC bias of captured libraries with 8-plex.