Total Solution for MGI Platforms

Background

Nanodigmbio provides NGS total solution on MGI platforms, including library preparation and hybridization capture.

Solution

MGI捕获应用-流程图

	Library preparation	Blocking	Target capture
Exome sequencing	NadPrep DNA Universal Library Preparation Kit (for MGI)	NadPrep NanoBlockers (for MGI)	Exome Plus Panel
Exome sequencing	To learn more, visit Whole Exome Sequencing solution
Solid tumor analysis	NadPrep DNA Universal Library Preparation Kit (for MGI)	NadPrep NanoBlockers (for MGI)	NanOnco Plus Panel NanOnCT Panel LungCancer Panel
Hematologic malignancy analysis	NadPrep DNA Universal Library Preparation Kit (for MGI)	NadPrep NanoBlockers (for MGI)	NanoHema Research Panel
Low-frequency mutation analysis	NadPrep cfDNA Library Preparation Kit (for MGI)	NadPrep NanoBlockers (for MGI)	Nan OnCT Panel
Low-frequency mutation analysis	To learn more, visit Low-frequency Mutation Analysis solution

Case Study: Exome sequencing

Fig 1. Capture performance of exome sequencing. The gDNA and FFPE libraries were prepared using NadPrep library kits for MGI. Exome Plus Panel v2.0 was used for target enrichment. The enriched libraries were sequenced on DNBSEQ-G400 (PE150) and subsampled to 35 M read pairs for the analysis of A. mappablity, B. on-target rate and C. coverage uniformity.

Case Study: Solid tumor analysis

Table 1. Performance of the NanOnCT Panel v1.0

	Mappability (%)	On-target rate (%)	Duplication (%)
gDNA-1	99.75	87.32	2.91
gDNA-2	99.81	85.28	2.91

gDNA-1 is Onco Structural Multiplex 5% gDNA (GeneWell, GW-OGTM001); gDNA-2 is Onco SNV1-25% gDNA (GeneWell, GW-OGTM004). The libraries are prepared using NadPrep library kits for MGI. Targeted regions were enriched by NanOnCT Panel and 1 M read pairs were subsampled for analysis.