Don’t Be the Blood-Extraction Sucker: NadPure Helps You Get High-Quality gDNA

One Blood Sample, Many Challenges:

What Makes Blood gDNA Extraction So Demanding?

Reliable DNA extraction powered by high-binding-capacity superparamagnetic beads and a

specially optimized buffer system.

01 Introduction

NadPure Blood Genomic DNA Kit utilizes high-binding superparamagnetic beads and a specially optimized buffer system to rapidly isolate and purify high-quality genomic DNA from 50-1,000 μL of fresh or frozen blood samples containing various anticoagulants.The kit provides a safe and convenient extraction workflow without the use of toxic reagents such as phenol or chloroform. It effectively removes proteins, inorganic salts, and other contaminants while maintaining high nucleic acid recovery.

The purified nucleic acids can be directly used for a variety of downstream applications, including PCR, qPCR, RT-PCR, Southern blotting, genotyping, and the library preparation of next-generation sequencing.

Figure 1. Workflow of gDNA extraction using the NadPure Blood Genomic DNA Kit.

02 Features

03 Performance

I. Flexible Compatibility Across Sample Input Volumes

The NadPure Blood Genomic DNA Kit supports the extraction of genomic DNA from 50-1,000 μL of blood samples, and the resulting eluate can be used directly in downstream applications without additional purification.

Figure 2. Performance of the NadPure Blood Genomic DNA Kit for gDNA extraction form the whole blood at different input volumes. A. DNA yield; B. DNA purity; C. Agarose gel electrophoresis of extracted gDNA (100 ng loaded per lane; the same loading amount applies to subsequent figures unless otherwise specified).

II. Broad Compatibility with Samples of Varying Quality

Whether using fresh whole blood (FWB) or hemolyzed whole blood (HWB), the NadPure Blood Genomic DNA Kit demonstrates gDNA extraction performance comparable to competing products, and achieves superior A260/230 ratios.

Figure 3. Comparison of the extraction performance among different magnetic bead-based blood gDNA extraction kits using various types of blood samples. A. DNA yield; B. DNA purity; C. Agarose gel electrophoresis of extracted gDNA. gDNA was extracted from fresh and hemolyzed whole blood samples using the NadPure Blood Genomic DNA Kit and comparable kits from Vendor A & B, respectively.

Note: The initial sample input volume was 200 μL for all samples.

III. Reliable Capability Against Diverse Interfering Substances

The NadPure Blood Genomic DNA Kit demonstrates excellent anti-interference capabilities against common endogenous and exogenous interfering substances in blood, effectively reducing the impact of interfering substances on gDNA purity.

Figure 4. NadPure Blood Genomic DNA Kit Performance of the NadPure Blood Genomic DNA Kit for blood gDNA extraction in the presence of various interfering substances. gDNA was extracted from simulated blood samples containing various endogenous and exogenous interfering substances using the NadPured Blood Genomic DNA Kit, and the quality of the extracted products was evaluated. A. DNA yield; B. DNA purity; C. Agarose gel electrophoresis of extracted gDNA.

Note: Based on relevant NMPA/FDA review and guidance documents and authoritative international literature, interfering substances were added at concentrations considered clinically relevant. For each group, 100 μL of whole blood was used as the initial sample, and the corresponding amount of each interfering substance was added. The volume was then brought to 200 μL with 1X PBS, followed by gDNA extraction according to the kit’s standard operating procedure. TG: Triglycerides; BIL: Bilirubin; Hb: Hemoglobin; DC: Disodium citrate; SH: Sodium heparin; SIM: Simvastatin.

IV. Consistent Performance Across Manufacturing Batches

Leveraging Nanodigmbio’s robust quality management system, the NadPure Blood Genomic DNA Kit demonstrates excellent extraction consistency across different batches, with an inter-batche CV well below 15%, ensuring stable and reliable product performance.

Figuer 5. NadPure Blood Genomic DNA Kit demonstrates good consistency in gDNA extraction from blood samples across different batches. A. DNA yield; B. DNA purity; C. Agarose gel electrophoresis of extracted gDNA.

Note: The initial sample input volume was 200 μL for all samples.

V. High-Quality Nucleic Acids for Downstream Applications

The NGS targeted capture library prepared using gDNA extracted by the NadPure Blood Genomic DNA Kit exhibited performance comparable to other similar products in all basic quality control indicators, meeting the needs of downstream targeted sequencing applications.

Figure 6. NadPure Blood Genomic DNA Kit consistently yields high-quality nucleic acids that meet the requirements for NGS capture library preparation, even in the presence of different interfering substances. A.Mappability and On-target rate; B. Target covered; C. Fold 80 base penalty; D. GC bias. gDNA was extracted from simulated blood samples containing various endogenous and exogenous interfering substances using the NadPure Blood Genomic DNA Kit. Pre-libraries were prepared using the NadPrep EZ DNA Library Preparation Kit v3, followed by hybridization capture using the NadPrep ES Hybrid Capture Reagents v2 and the NanOnco Plus Panel v3.0. Raw reads were aligned to the human reference genome using BWA. On-target rate was calculated by the number of reads. Sequencing was performed on the NovaSeq 6000, PE150.

04 Summary and Outlook

Newly launched by Nanodigmbio, the NadPure Blood Genomic DNA Kit utilizes high-binding superparamagnetic beads and a specially optimized buffer system to efficiently extract high-purity gDNA from 50-1,000 μL of fresh, frozen, or anticoagulant-treated blood samples in just 55 minutes, without requiring large centrifugation equipment. The purified gDNA provides high-quality templates for a variety of downstream molecular applications, including PCR, genotyping, and NGS.

Furthermore, the NadPure Blood Genomic DNA Kit is compatible with fully automated nucleic acid extraction instruments, further reducing manual handling while enhancing sample processing efficiency and extraction throughput. It offers a more flexible, efficient, and reliable solution for blood gDNA extraction across diverse application scenarios.