NadPrep EZ RNA Library Preparation Kit is widely suitable for the libraries prepared from total RNA with different grades, poly(A)-enriched mRNA, and rRNA-depleted RNA. It should be noted that the mRNA content in total RNA from different sources varies greatly. If the initial input amount of total RNA is too low, sufficient mRNA may not be obtained to prepare a high-quality library.

RNA-Seq library preparation generally falls into two types: non-stranded (general) and stranded (strand-specific). The core difference is whether the library retains transcript directional information. Among them, stranded libraries retain the transcript directional information during the preparing process, and sequencing and downstream analysis can determine whether the transcripts originate from the sense or antisense strands of DNA. However, non-stranded libraries lose directional information during cDNA synthesis, and sequencing data cannot directly indicate transcript directions. Compared with general RNA-Seq, strand-specific RNA-Seq provides more accurate transcript quantification, clearer gene structures, identification of antisense transcripts, and discovery of novel transcripts. Therefore, stranded RNA-Seq is crucial for studies of gene structure and expression regulation. To obtain transcript direction information, stranded libraries should be preferred.

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.

Figure 2. Correlation of ERCC expression profiles in captured libraries from RNA samples processed through different library preparation protocols. A. NadPrep RNA & DNA Library Co-Preparation Module; B. NadPrep Total RNA-To-DNA Module conjunction NadPrep DNA Library Preparation Module.

Note: The samples originated from K562 cell line RNA with an initial input of 50 ng; the pre-library input amount was 200 ng.

Figure 1. Library yield and capture performance of DNA samples using different library preparation protocols. A. Library Yield; B. Mappability.

Note: The sample originated from 50 ng of Human Genomic DNA Standard (Promega, G1521). Sequencing mode was Illumina NovaSeq 6000 platform, PE150.

Libraries prepared with this module can be used in conjunction with NEX-t Panel v1.0 and NadPrep ES Hybrid Capture Reagents. This significantly shortens the time required for pathogen-targeted sequencing and simplifies experimental procedures.